![]() The resulting mRNA could not be transcribed. Target guides were designed for either end of region including exons 1–13 to ensure complete deletion. Briefly, ADSS is predicted to have three transcripts and exons 1–13 (AA 1-456) encode the protein. ADSS knockout is lethal therefore, heterozygous mice were established spCas91.1 technology (ViewSolid Biotech, Beijing, China). ![]() Male WT C57BL/6 J mice at 8 weeks old and obese db/db mice at 8 and 24 weeks old were used in the study. The protocols for mice experiments were approved by the ethics committees of Beijing Youan Hospital. LDL cholesterol (LDL-c) was calculated from serum total and HDL cholesterol (HDL-c) and serum triglycerides by the Friedewald equation 19. Biochemical measurementĪfter overnight fasting, venous blood samples were drawn to measure total and differential white blood cell counts, serum cholesterol and triglycerides, HDL cholesterol, HbA1c, uric acid levels, and plasma glucose levels. Additional characteristics including age, body weight, height, systolic blood pressure, diastolic blood pressure, heart rate, disease history and medications were recorded. Hypertension was defined as blood pressure of ≥140 mmHg systolic, 90 mmHg diastolic, or the use of antihypertensive drugs. Diabetes was defined as either fasting plasma glucose levels ≥7.0 mmol/L or glucose levels ≥11.0 mmol/L at 2 h after an orally administered glucose load of 75 g. Type 2 diabetic patients with or without AP were enrolled in the Department of Cardiology of Yangzhou hospital, the Department of Endocrinology of Beijing TongRen hospital, and the Department of Science and Technology of Beijing Youan hospital from March 2016 to May 2017. All the patients provided written informed consent. ![]() The study complied with the Helsinki Declaration for the investigation of human subjects and received ethical approval from the competent Institutional Review Boards of the Beijing Youan Hospital and Capital Medical University. ![]()
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